National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Anyang, Republic of Korea.
Vet Microbiol. 2011 Mar 24;148(2-4):396-401. doi: 10.1016/j.vetmic.2010.08.035. Epub 2010 Sep 8.
Five coronaviruses (CoVs) were detected in diarrheal feces from four zoo ruminant species: one wisent (Bison bonasus), two Himalayan tahr (Hemitragus jemlahicus), one sitatunga (Tragelaphus spekii), and one nyala (Tragelaphus angasii). We sequenced and analyzed the spike (S) and hemagglutinin/esterase (HE) genes of these viruses and compared the nucleotide (nt) and deduced amino acid (aa) sequences with those of other bovine CoV (BcoV) strains. Comparison of the entire deduced aa sequences of the S and HE glycoproteins revealed no specific differences that would account for discrimination between bovine-like CoV strains from zoo ruminants and BcoVs strains. In addition, the 99.9% aa identity among the five CoV strains revealed that the ruminants were infected by the same strain. Phylogenetically, bovine-like CoVs belong to group 2a CoVs, which are related most closely to the BcoV strains recently isolated in Korea. These data suggest that cattle are potential reservoirs for CoVs that are capable of infecting zoo ruminants.
从四种动物园反刍动物的腹泻粪便中检测到了五种冠状病毒（CoV）：一头白野牛（Bison bonasus）、两头喜马拉雅塔尔羊（Hemitragus jemlahicus）、一头转角牛羚（Tragelaphus spekii）和一头黇鹿（Tragelaphus angasii）。我们对这些病毒的刺突（S）和血凝素/酯酶（HE）基因进行了测序和分析，并将核苷酸（nt）和推导的氨基酸（aa）序列与其他牛冠状病毒（BcoV）株进行了比较。S 和 HE 糖蛋白的整个推导 aa 序列比较表明，没有特定的差异可以区分动物园反刍动物中的牛样 CoV 株和 BcoV 株。此外，这五种 CoV 株之间 99.9%的 aa 同一性表明，这些反刍动物被同一株病毒感染。系统进化分析表明，牛样 CoV 属于 2a 组 CoV，与最近在韩国分离的 BcoV 株关系最密切。这些数据表明，牛可能是能够感染动物园反刍动物的 CoV 的潜在宿主。
