Morais Sergi, Carrascosa Javier, Mira David, Puchades Rosa, Maquieira Angel
Departamento de Química, Instituto de Química Molecular Aplicada, and Centro de Tecnología Nanofotónica de Valencia, Universidad Politécnica de Valencia, Camino de Vera s/n, 46071 Valencia, Spain.
Anal Chem. 2007 Oct 15;79(20):7628-35. doi: 10.1021/ac070328b. Epub 2007 Sep 14.
High-density competitive indirect microimmunoassays were performed in both sides of compact discs by direct absorption of immunoreagents on polycarbonate surface, using gold- or enzyme-labeled immunoglobulins as tracers for displaying the immunoreaction. The operational principle is based on the use of a low-reflectivity compact disc as analytical platform that allows the reflection/transmission (30/70%) of the CD reader laser beam (lambda 780 nm). The reflected light is used to scan the disc track keeping it in movement. The transmitted light is detected by a planar photodiode integrated on the CD drive. The variation of the optical transmission of the light caused by the immunoreaction products is related to the sample concentration. As a proof of concept, low abundant compounds, commonly used as pesticides, were detected in a 60-min total assay time, with a limit of detection ranging from 0.02 to 0.62 microg/L for 2,4,5-TP, chlorpyriphos, and metolachlor. The obtained results show the enormous prospective of compact discs in combination with CD players for multiresidue and drug discovery applications.
通过将免疫试剂直接吸附在聚碳酸酯表面，在光盘的两面进行高密度竞争性间接微免疫测定，使用金标记或酶标记的免疫球蛋白作为示踪剂来显示免疫反应。其操作原理基于使用低反射率光盘作为分析平台，该平台允许CD读取器激光束（波长780nm）进行30%反射/70%透射。反射光用于扫描光盘轨道并使其保持移动。透射光由集成在CD驱动器上的平面光电二极管检测。免疫反应产物引起的光的光透射变化与样品浓度相关。作为概念验证，在60分钟的总测定时间内检测到了常用作农药的低丰度化合物，对于2,4,5-涕、毒死蜱和异丙甲草胺，检测限范围为0.02至0.62μg/L。所得结果表明，光盘与CD播放器结合在多残留和药物发现应用方面具有巨大的前景。
