Wood P M
Eur J Biochem. 1978 Jun 1;87(1):9-19. doi: 10.1111/j.1432-1033.1978.tb12346.x.
The interrelation of the copper protein plastocyanin, and a soluble c-type cytochrome, c-552, in photosynthetic electron transport has been studied in the genus Chlamydomonas. With C. reinhardtii the plastocyanin: cytochrome c-552 ratio could be changed from 300:1 less than 1:16 simply by omitting copper from the medium, without any other detectable change. Plastocyanin was indetectable in a second species, C. mundana, for which the cytochrome c-552 level was always very high. The properties of Levine's C. reinhardtii mutant lacking plastocyanin, ac-208, were studies and it was found that the photosynthetic capabilities of a suppressed phenotype and suppressed genotype could be explained by reference to the cytochrome c-552 levels. Both proteins were successfully used in reconstitution experiments with chloroplast fragments. Both showed very fast kinetics for reduction by purified Chlamydomonas cytochrome f, but the rate of electron transfer from one to the other was much slower. It is concluded that they constitute an interchangeable pair, and the rationale for this and possible analogies are both discussed.
在衣藻属中研究了铜蛋白质体蓝素与一种可溶性c型细胞色素c - 552在光合电子传递中的相互关系。对于莱茵衣藻，仅通过从培养基中去除铜，质体蓝素与细胞色素c - 552的比例就可以从300:1变为小于1:16，且没有任何其他可检测到的变化。在第二种衣藻——蒙氏衣藻中未检测到质体蓝素，而其细胞色素c - 552水平一直非常高。对莱文的缺乏质体蓝素的莱茵衣藻突变体ac - 208的特性进行了研究，发现可以通过细胞色素c - 552水平来解释抑制表型和抑制基因型的光合能力。这两种蛋白质都成功地用于叶绿体片段的重组实验。它们在被纯化的衣藻细胞色素f还原时均表现出非常快的动力学，但从一种蛋白质到另一种蛋白质的电子传递速率要慢得多。得出的结论是，它们构成了一对可互换的物质，并对此的基本原理以及可能的类比进行了讨论。
