Zhen Zhang-He, Qin Song, Ren Qing-Min, Wang Yu, Ma Yu-Ying, Wang Yin-Chu
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
University of Chinese Academy of Sciences, Beijing, China.
Front Bioeng Biotechnol. 2021 May 10;9:673402. doi: 10.3389/fbioe.2021.673402. eCollection 2021.
Cyanobacteria can acclimate to changing copper and iron concentrations in the environment via metal homeostasis, but a general mechanism for interpreting their dynamic relationships is sparse. In this study, we assessed growth and chlorophyll fluorescence of sp. PCC 6803 and investigated proteomic responses to copper and iron deductions. Results showed that copper and iron exerted reciprocal effect on the growth and photosynthesis of sp. PCC 6803 at combinations of different concentrations. And some proteins involved in the uptake of copper and iron and the photosynthetic electron transport system exhibit Cu-Fe proteomic association. The protein abundance under copper and iron deduction affected the photosynthetic electronic activity of sp. PCC 6803 and eventually affected the growth and photosynthesis. Based on these results, we hypothesize that the Cu-Fe proteomic association of sp. PCC 6803 can be elucidated via the uptake system of outer membrane-periplasmic space-inner plasma membrane-thylakoid membrane, and this association is mainly required to maintain electron transfer. This study provides a broader view regarding the proteomic association between Cu and Fe in cyanobacteria, which will shed light on the role of these two metal elements in cyanobacterial energy metabolism and biomass accumulation.
蓝细菌可以通过金属稳态来适应环境中不断变化的铜和铁浓度，但解释它们动态关系的一般机制尚不清楚。在本研究中，我们评估了聚球藻属PCC 6803的生长和叶绿素荧光，并研究了对铜和铁减少的蛋白质组学反应。结果表明，在不同浓度组合下，铜和铁对聚球藻属PCC 6803的生长和光合作用产生相互影响。并且一些参与铜和铁摄取以及光合电子传递系统的蛋白质表现出铜 - 铁蛋白质组学关联。铜和铁减少条件下的蛋白质丰度影响了聚球藻属PCC 6803的光合电子活性，最终影响了生长和光合作用。基于这些结果，我们推测聚球藻属PCC 6803的铜 - 铁蛋白质组学关联可以通过外膜 - 周质空间 - 内膜 - 类囊体膜的摄取系统来阐明，并且这种关联主要是为了维持电子传递。本研究为蓝细菌中铜和铁之间的蛋白质组学关联提供了更广阔的视角，这将有助于揭示这两种金属元素在蓝细菌能量代谢和生物量积累中的作用。
