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质体蓝素与细胞色素c-553的相互形成以及铜离子对光合电子传递的影响。

Reciprocal formation of plastocyanin and cytochrome c-553 and the influence of cupric ions on photosynthetic electron transport.

作者信息

Bohner H, Böhme H, Böger P

出版信息

Biochim Biophys Acta. 1980 Aug 5;592(1):103-12. doi: 10.1016/0005-2728(80)90117-6.

DOI:10.1016/0005-2728(80)90117-6
PMID:6249351
Abstract

The green alga Scenedesmus acutus is able to synthesize plastocyanin and cytochrome c-553. The concentrations of plastocyanin and cytochrome c-553 vary inversely in response to the cupric-ion concentrations of the growth medium (Bohner, H. and Böger, P. (1978) FEBS Lett. 85, 337-339). Both proteins form a homogeneous donor pool to the reaction center of Photosystem I. This donor pool can be varied quantitatively and qualitatively by different growth conditions. These variations have no influence on algal growth or photosynthetic electron transport as measured in vivo by oxygen evolution, fluorescence induction and cytochrome f-553 and c-553 redox reactions using Cu2+ concentrations of less than 10 microM in the culture medium. At higher cupric-ion concentrations, which already retard algal growth, specific sites of the photosynthetic electron-transport chain are affected: the oxidizing side of Photosystem II and the reducing side of Photosystem I.

摘要

绿藻尖细栅藻能够合成质体蓝素和细胞色素c-553。质体蓝素和细胞色素c-553的浓度随生长培养基中铜离子浓度的变化呈反比(博纳,H.和伯格尔,P.(1978年)《欧洲生物化学学会联合会快报》85,337 - 339)。这两种蛋白质形成一个向光系统I反应中心的同质供体库。该供体库可通过不同的生长条件在数量和质量上发生变化。在培养基中铜离子浓度低于10微摩尔时,通过体内放氧、荧光诱导以及细胞色素f-553和c-553氧化还原反应测量,这些变化对藻类生长或光合电子传递没有影响。在已经阻碍藻类生长的较高铜离子浓度下,光合电子传递链的特定部位会受到影响:光系统II的氧化侧和光系统I的还原侧。

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