葡萄糖可提高成年果蝇胰岛素生成细胞的活性并增加细胞内钙离子浓度。
Glucose increases activity and Ca2+ in insulin-producing cells of adult Drosophila.
作者信息
Kréneisz Orsolya, Chen Xinnian, Fridell Yih-Woei C, Mulkey Daniel K
机构信息
Departments of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269, USA.
出版信息
Neuroreport. 2010 Dec 8;21(17):1116-20. doi: 10.1097/WNR.0b013e3283409200.
Abstract
We sought to understand the mechanisms underlying glucose sensing in Drosophila melanogaster. We found that insulin-producing cells (IPCs) of adult Drosophila respond to glucose and glibenclamide with a burst-like pattern of activity. Under controlled conditions IPCs have a resting membrane potential of -62+/-4 mV. In response to glucose or glibenclamide, IPCs generate action potentials at a threshold of -36+/-1.4 mV with an amplitude of 46+/-4 mV and width of 9.3+/-1.8 ms. Real-time Ca imaging confirms that IPCs respond to glucose and glibenclamide with increased intracellular Ca. These results provide the first detailed characterization of electrical properties of IPCs of adult Drosophila and suggest that these cells sense glucose by a mechanism similar to mammalian pancreatic β cells.
摘要
我们试图了解黑腹果蝇中葡萄糖感知的潜在机制。我们发现，成年果蝇的胰岛素分泌细胞（IPCs）对葡萄糖和格列本脲会产生爆发样的活动模式反应。在受控条件下，IPCs的静息膜电位为-62±4 mV。响应葡萄糖或格列本脲时，IPCs在阈值-36±1.4 mV时产生动作电位，幅度为46±4 mV，宽度为9.3±1.8 ms。实时钙成像证实，IPCs对葡萄糖和格列本脲的反应是细胞内钙增加。这些结果首次详细描述了成年果蝇IPCs的电特性，并表明这些细胞通过类似于哺乳动物胰腺β细胞的机制感知葡萄糖。
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