Markovic O, Jörnvall H
Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden.
Protein Seq Data Anal. 1990 Dec;3(6):513-5.
Existing reports on the enzyme tomato pectinesterase differ in alignments of two internal segments and in many single-residue replacements. The alignment from cDNA data, with a 317-residue mature protein, is correct, but protein analyses also show at least 18 single-residue replacements, suggesting the presence of many native or (less likely) cloning-derived microheterogeneities. Purification of Aspergillus niger pectinesterase and N-terminal sequence analysis of this enzyme reveal a different structure and indicate an extensive protein divergence.
现有关于番茄果胶酯酶的报告在两个内部片段的比对以及许多单残基替换方面存在差异。来自cDNA数据的比对结果显示，成熟蛋白有317个残基，这是正确的，但蛋白质分析也显示至少有18个单残基替换，这表明存在许多天然的或（可能性较小的）克隆衍生的微异质性。黑曲霉果胶酯酶的纯化及其N端序列分析揭示了一种不同的结构，并表明存在广泛的蛋白质差异。
