从黑曲霉（无花果曲霉）中鉴定并克隆第二个植酸酶基因（phyB）。
Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum).
作者信息
Ehrlich K C, Montalbano B G, Mullaney E J, Dischinger H C, Ullah A H
机构信息
Southern Regional Research Center, United States Department of Agriculture, New Orleans, LA 70179.
出版信息
Biochem Biophys Res Commun. 1993 Aug 31;195(1):53-7. doi: 10.1006/bbrc.1993.2008.
An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino acid enzyme was isolated and found to contain four exons. Global alignment revealed 23.5% homology to Aspergillus niger phytase (PhyA); four regions of extensive homology were identified. Some of these regions may contain catalytic sites for phosphatase function.
用一个354碱基对的DNA片段探测黑曲霉（ficuum）基因组DNA λEMBL3文库，该片段是通过基于最适pH 2.5的酸性磷酸酶部分氨基酸序列的寡核苷酸对黑曲霉DNA进行聚合酶链反应获得的。分离出一个含有编码479个氨基酸的酶的1605碱基对片段（phyB）的克隆，发现其含有四个外显子。全局比对显示与黑曲霉植酸酶（PhyA）有23.5%的同源性；鉴定出四个广泛同源的区域。这些区域中的一些可能包含磷酸酶功能的催化位点。
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