Hacham H, Freeman S E, Gange R W, Maytum D J, Sutherland J C, Sutherland B M
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
Photochem Photobiol. 1990 Oct;52(4):893-6. doi: 10.1111/j.1751-1097.1990.tb08698.x.
A previous report [Freeman et al. (1986) Photochem. Photobiol. 43S, 93S] indicated that irradiation of human skin in situ with 385 or 405 nm radiation produced detectable levels of pyrimidine dimers in DNA. Since these wavelengths are absorbed poorly by DNA, these results suggested that DNA damage was sensitized by other absorbing molecules present in skin. Examination of two experimental aspects of the previous work indicates that (1) the static gel electrophoresis method for DNA dispersion used in lesion determination gave accurate values of the levels of induced dimers, and (2) the DNA damage apparently induced by 385 nm was actually induced by shorter wavelength UV present in the 20 nm bandpass beam of the monochromator. The current results indicate that monochromatic 385 and 405 nm radiation are ineffective in dimer production in human skin in situ.
先前的一份报告[弗里曼等人（1986年），《光化学与光生物学》43S，93S]表明，用385或405纳米辐射对人体皮肤进行原位照射，会在DNA中产生可检测水平的嘧啶二聚体。由于这些波长被DNA吸收的能力较差，这些结果表明，DNA损伤是由皮肤中存在的其他吸收分子致敏的。对先前工作的两个实验方面进行检查表明：（1）用于损伤测定的DNA分散静态凝胶电泳方法给出了诱导二聚体水平的准确值；（2）显然由385纳米诱导的DNA损伤实际上是由单色仪20纳米带通光束中存在的较短波长紫外线诱导的。目前的结果表明，单色385和405纳米辐射在人体皮肤原位产生二聚体方面是无效的。
