Fernández Andreu C, Vilarrubia Montes de Oca O L, Martínez Machín G, Oramas Rodríguez B
Instituto de Medicina Tropical Pedro Kouri, Laboratorio de Micología.
Rev Cubana Med Trop. 1990 May-Aug;42(2):272-8.
The exoantigen test has been successfully used in the last years for the immunological identification of pathogenic fungi. Twenty seven straing presumptively identified as Histoplasma capsulatum by morphologic methods, were studied by such test. The exoantigens obtained from each strain were studied by immunoprecipitation technique in front of histoplasmosis control sera experimentally obtained in rabbits. Reference strains of Histoplasma capsulatum. Histoplasma duboisii and Paracoccidioides brasiliensis were used. Of the strains studied, 96% was identified as H. capsulatum by the exoantigen test, a simple, rapid and highly specific technique greatly useful in the laboratories of Microbiology.
外抗原试验在过去几年已成功用于致病真菌的免疫学鉴定。通过形态学方法初步鉴定为荚膜组织胞浆菌的27株菌株,采用该试验进行了研究。从各菌株获得的外抗原,用免疫沉淀技术在实验兔身上获得的组织胞浆菌病对照血清前进行研究。使用了荚膜组织胞浆菌、杜波伊斯组织胞浆菌和巴西副球孢子菌的参考菌株。在所研究的菌株中,96%通过外抗原试验鉴定为荚膜组织胞浆菌,这是一种简单、快速且高度特异的技术,在微生物学实验室非常有用。