Gaffney E V, Pigott D
In Vitro. 1978 May;14(5):451-7. doi: 10.1007/BF02616107.
Clusters of cells derived from biopsy specimens of human mammary ductal carcinomas form two morphologically distinct epithelial colonies in culture, designated as E and E'. The proportion of E' cell clusters that attached and formed colonies ranged from 0.3 to 13.0% with different tumors. Attachment was independent of tumor grade. Microscopic observations revealed that the survival of E' cell colonies was limited to approximately 10 days with rapid cell degeneration commencing about 7 days. A comparison of sera showed that colony formation by cells from malignant tumors during the 1st week of culture was maximum in the presence of fetal bovine serum. Human serum alone was 70 to 100% less effective in promoting E' colonies. The most significant finding was that human serum from normal donors inhibited E' colony development in the presence of FBS. Although human serum was less effective than FBS in promoting colony formation by clusters of E cells, an inhibition was not observed. Inhibitory activity could not be attributed to either antagonistic hormones or the source of human serum. These results demonstrate that normal human serum contains a factor(s) that exhibits an inhibitory activity specific for human epithelial cells (E') derived from malignant tumors.
从人乳腺导管癌活检标本中获取的细胞簇在培养中形成两种形态上不同的上皮菌落，分别命名为E和E'。不同肿瘤来源的E'细胞簇附着并形成菌落的比例在0.3%至13.0%之间。附着与肿瘤分级无关。显微镜观察显示，E'细胞菌落的存活期约为10天，约7天后细胞开始迅速退化。血清比较表明，在培养的第1周，恶性肿瘤细胞在胎牛血清存在下形成菌落的情况最为明显。单独的人血清在促进E'菌落形成方面的效果要低70%至100%。最显著的发现是，正常供体的人血清在胎牛血清存在下会抑制E'菌落的发育。虽然人血清在促进E细胞簇形成菌落方面不如胎牛血清有效，但未观察到抑制作用。抑制活性既不能归因于拮抗激素，也不能归因于人血清的来源。这些结果表明，正常人血清中含有一种对源自恶性肿瘤的人上皮细胞（E'）具有特异性抑制活性的因子。
