Gaffney E V, Pigott D
In Vitro. 1978 Jul;14(7):621-4. doi: 10.1007/BF02617922.
Rapid proliferation of mammary epithelial cells derived from biopsy specimens of human fibroadenomas was observed when medium was supplemented with ten percent fetal bovine serum and hydrocortisone (5 microgram per ml-1). Hydrocortisone in combination with FBS also led to a 2.5-fold increase in cell cluster attachment and subsequent colony formation. A similar effect was not observed with human serum. In contrast to fibroblast cell systems, insulin did not significantly alter cell growth. The results show that a mitogenic response to glucocorticoids by mammary epithelium may depend on the presence of factors in sera.
当培养基中添加10%的胎牛血清和氢化可的松（每毫升5微克）时，观察到源自人纤维腺瘤活检标本的乳腺上皮细胞迅速增殖。氢化可的松与胎牛血清联合使用还使细胞簇附着和随后的集落形成增加了2.5倍。用人血清未观察到类似效果。与成纤维细胞系统不同，胰岛素并未显著改变细胞生长。结果表明，乳腺上皮细胞对糖皮质激素的促有丝分裂反应可能取决于血清中某些因子的存在。
