de Micco C, Tripier M F, Hassoun J, Lipcey C, Meyer G, Toga M
Int J Cancer. 1978 Apr 15;21(4):516-22. doi: 10.1002/ijc.2910210419.
The transformation of cultivated hamster brain cells by polyoma virus is reported. The transformed cell line contained polyoma virus-specific nuclear, surface and transplantation antigens. Subcutaneous and intracranial inoculations revealed high tumorigenicity of the cells. Brain-specific S 100 protein was found in these tumors with immuno-peroxidase staining, suggesting that they were of a nervous nature. Both in vivo and in vitro, the cells had glial features as studied by phase contrast, light and electron microscopy. Type-H virus-like particles were found in the tumor cells and might have played a role in the viral transformation.
本文报道了多瘤病毒对培养的仓鼠脑细胞的转化作用。转化后的细胞系含有多瘤病毒特异性的核抗原、表面抗原和移植抗原。皮下和颅内接种显示这些细胞具有高度致瘤性。通过免疫过氧化物酶染色在这些肿瘤中发现了脑特异性S100蛋白，表明它们具有神经性质。通过相差显微镜、光学显微镜和电子显微镜研究发现，这些细胞在体内和体外均具有神经胶质细胞的特征。在肿瘤细胞中发现了H型病毒样颗粒，其可能在病毒转化过程中发挥了作用。
