用玻璃套管收集的继发性白内障物质。一项扫描电子显微镜研究。

Secondary cataract material collected with a glass cannula. A SEM study.

作者信息

Jongebloed W L, van der Veen G, Dijk F, Worst J G

机构信息

Department of Histology and Cellbiology, University of Groningen, The Netherlands.

出版信息

Doc Ophthalmol. 1990 Oct;75(3-4):359-64. doi: 10.1007/BF00164851.

DOI:10.1007/BF00164851

PMID:2090412

Abstract

Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.

摘要

分别在术后约18、24和30个月，用玻璃插管收集了3例患者的后发性白内障物质，准备用于扫描电子显微镜（SEM）检查。对于软性样本，通过微孔滤膜过滤，然后固定和干燥来进行处理。较硬的物质悬浮于固定液中，接着离心，再悬浮于70%乙醇中，并在样品架上干燥。术后18个月的短期留存样本主要显示为红细胞、一些（炎性）细胞和变性的晶状体纤维物质。大多数在术后20个月以上收集的较硬物质，显示出（再生的）囊膜上皮碎片以及附着有囊膜上皮碎片的固态晶状体纤维物质碎片。