地方性甲状腺肿组织中的甲状腺过氧化物酶。
Thyroid peroxidase in endemic goiter tissue.
作者信息
Sugawara M, Summer C N, Kobayashi A, Murakami S, Kuma K, Medeiros-Neto G A
机构信息
Endocrine Division, Wadsworth Veterans Administration Hospital, Los Angeles, California 90073.
出版信息
J Endocrinol Invest. 1990 Dec;13(11):893-9. doi: 10.1007/BF03349648.
Thyroid peroxidase (TPO) activity and TPO protein were analyzed in endemic goiter tissue under iodine deficiency and after iodine supplement. TPO was prepared from 9 endemic goiter tissues and 4 normal thyroid tissues by solubilizing enzyme with detergent. Four patients with endemic goiter received iodized oil injection 12 months before surgery. All patients had normal serum thyrotropin (TSH) and thyroid hormone levels before surgery. TPO activity was measured by iodinase assay and guaiacol assay. Endemic goiter TPO showed greater iodination activity than that of normal TPO (p less than 0.01). The guaiacol assay showed greater TPO activity in 6 of the 9 endemic goiter tissues than that of the normal tissue. Iodized oil treatment did not affect TPO activity or TPO proteins when compared with those in untreated endemic goiter tissues. TPO activity in endemic goiter tissue correlated with thyroid T4 5'-deiodinase activity and not with thyroid hormone content in thyroglobulin. Since thyroid T4 5'-deiodinase and TPO are under control of TSH, an increase in TPO activity in the presence of normal serum TSH may be explained by the increased sensitivity of endemic goiter tissue to TSH-one of the possible adaptation mechanisms of endemic goiter.
对碘缺乏及补碘后地方性甲状腺肿组织中的甲状腺过氧化物酶（TPO）活性和TPO蛋白进行了分析。通过用去污剂溶解酶，从9个地方性甲状腺肿组织和4个正常甲状腺组织中制备TPO。4例地方性甲状腺肿患者在手术前12个月接受了碘油注射。所有患者术前血清促甲状腺激素（TSH）和甲状腺激素水平均正常。通过碘酶法和愈创木酚法测定TPO活性。地方性甲状腺肿TPO的碘化活性高于正常TPO（p＜0.01）。愈创木酚法显示，9个地方性甲状腺肿组织中有6个的TPO活性高于正常组织。与未治疗的地方性甲状腺肿组织相比，碘油治疗不影响TPO活性或TPO蛋白。地方性甲状腺肿组织中的TPO活性与甲状腺T4 5'-脱碘酶活性相关，而与甲状腺球蛋白中的甲状腺激素含量无关。由于甲状腺T4 5'-脱碘酶和TPO受TSH控制，在血清TSH正常的情况下TPO活性增加可能是由于地方性甲状腺肿组织对TSH的敏感性增加所致，这是地方性甲状腺肿可能的适应机制之一。
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