豚鼠中由各种交叉反应性抗原诱导的葡萄膜炎。
Uveitis induced by various cross-reactive antigens in guinea pigs.
作者信息
Sasaki K, Sanui H, Inomata H
机构信息
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
Ophthalmic Res. 1990;22(5):330-6. doi: 10.1159/000267042.
In order to investigate possible immunopathogenic mechanisms in the recurrence of uveitis, cross-reactive proteins were tested for their capacity to induce experimental uveitis. Guinea pigs were immunized with porcine serum albumin (PSA) in complete Freund's adjuvant (CFA) by subcutaneous injection. Fourteen or 28 days after the immunization, PSA, bovine (BSA), sheep (SSA), equine (ESA), rabbit (RSA) serum albumin, bovine gamma globulin (BCG) or ovalbumin (OA) was injected into the vitreous. Uveitis occurred in the eyes injected with PSA, BSA, SSA, ESA or RSA, but not BGG or OA. Serum antibodies and erythematous delayed-type skin reactions against PSA, BSA, SSA, ESA and RSA were positive in animals immunized with PSA in CFA. In an adoptive transfer study, humoral and cellular immunity recognized cross-reactive antigens and uveitis developed. Once a guinea pig is sensitized, uveitis may occur or recur from subsequent intravitreal challenge by antigens that are not completely the same but have a cross-reactivity with the immunizing antigen.
为了研究葡萄膜炎复发中可能的免疫致病机制，对交叉反应蛋白诱导实验性葡萄膜炎的能力进行了检测。通过皮下注射，用猪血清白蛋白（PSA）加完全弗氏佐剂（CFA）对豚鼠进行免疫。免疫后14天或28天，将PSA、牛血清白蛋白（BSA）、羊血清白蛋白（SSA）、马血清白蛋白（ESA）、兔血清白蛋白（RSA）、牛γ球蛋白（BCG）或卵清蛋白（OA）注入玻璃体。注入PSA、BSA、SSA、ESA或RSA的眼睛发生了葡萄膜炎，但注入BGG或OA的眼睛未发生。在用PSA加CFA免疫的动物中，针对PSA、BSA、SSA、ESA和RSA的血清抗体及红斑迟发型皮肤反应呈阳性。在一项过继转移研究中，体液免疫和细胞免疫识别交叉反应抗原并引发了葡萄膜炎。豚鼠一旦致敏，后续通过与免疫抗原不完全相同但有交叉反应的抗原进行玻璃体内激发，可能会发生葡萄膜炎或使其复发。
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