Wroblewska Z, Wellish M, Gilden D H
J Med Virol. 1978;2(2):127-36. doi: 10.1002/jmv.1890020207.
The ability of cytochalasin D (CD) and dimethyl sulfoxide (DMSO) to enhance parainfluenza I (6/94) virus replication was studied in various cell culture systems. Treatment of CV1 cells with CD (1 microgram/ml) dissolved in DMSO prior to primary 6/94 virus exposure at 10(0)--10(5) multiplicities of infection did not substantially enhance virus replication. However, there was a transient increase in cell associated virus one day after infection of DMSO-treated cultures. CD treatment of cultures of human brain cells latently infected with 6/94 virus (LIHB cells) did not enhance 6/94 virus detection. Cocultivation of CV1 cells with CD-treated LIHB cell cultures, and cocultivation of LIHB cell cultures with CD-treated CV1 cells, resulted in the production of both cell-associated and cell-free 6/94 virus three and five days after cocultivation. No virus was detected after similar cocultivation of untreated LIHB cell cultures with untreated CV1 cells. The usefulness of CD-DMSO treatment in the rescue of virus from 6/94 LIHB cell cultures appears limited to a cocultivation system. The use of these techniques to enhance virus rescue from human tissues suspected of harboring latent viral genomes is discussed.
在多种细胞培养系统中研究了细胞松弛素 D（CD）和二甲基亚砜（DMSO）增强 I 型副流感病毒（6/94）复制的能力。在以 10(0)--10(5)感染复数初次暴露于 6/94 病毒之前，用溶解于 DMSO 中的 CD（1 微克/毫升）处理 CV1 细胞，并未显著增强病毒复制。然而，在感染 DMSO 处理过的培养物一天后，细胞相关病毒有短暂增加。用 CD 处理潜伏感染 6/94 病毒的人脑细胞培养物（LIHB 细胞）并未增强 6/94 病毒的检测。将 CV1 细胞与经 CD 处理的 LIHB 细胞培养物共培养，以及将 LIHB 细胞培养物与经 CD 处理的 CV1 细胞共培养，在共培养三天和五天后均产生了细胞相关和游离的 6/94 病毒。未经处理的 LIHB 细胞培养物与未经处理的 CV1 细胞进行类似共培养后未检测到病毒。CD-DMSO 处理从 6/94 LIHB 细胞培养物中拯救病毒的作用似乎仅限于共培养系统。讨论了使用这些技术从疑似携带潜伏病毒基因组的人体组织中增强病毒拯救的问题。
