Fichaux F, Catala J, Bonnafous R
Laboratoire de Physiologie de la Nutrition, Université Paul Sabatier, Toulouse, France.
Diabetes Res. 1990 Feb;13(2):95-100.
This work describes a perfusion technique adapted to the isolated rabbit pancreas allowing investigation of both the endocrine and exocrine function. The pancreas-duodenum-stomach-spleen complex is removed and perfused with a modified Krebs Ringer Bicarbonate medium. The surgical steps leading to the removal of the complex are described. The endocrine response is studied by measuring insulin release when the pancreas is submitted to successive glucose stimulations and the exocrine function is evaluated by the alpha-chymotrypsin activity of the pancreatic juice harvested during the perfusion.
这项工作描述了一种适用于离体兔胰腺的灌注技术，可用于研究内分泌和外分泌功能。将胰腺-十二指肠-胃-脾复合体切除，并用改良的krebs ringer碳酸氢盐培养基进行灌注。描述了切除该复合体的手术步骤。通过测量胰腺在连续葡萄糖刺激下的胰岛素释放来研究内分泌反应，并通过灌注期间收集的胰液的α-糜蛋白酶活性来评估外分泌功能。
