Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA.
Tissue Eng Part A. 2011 Mar;17(5-6):631-44. doi: 10.1089/ten.tea.2010.0298. Epub 2010 Nov 17.
New strategies for skin regeneration are needed to address the significant medical burden caused by cutaneous wounds and disease. In this study, pullulan-collagen composite hydrogel matrices were fabricated using a salt-induced phase inversion technique, resulting in a structured yet soft scaffold for skin engineering. Salt crystallization induced interconnected pore formation, and modification of collagen concentration permitted regulation of scaffold pore size. Hydrogel architecture recapitulated the reticular distribution of human dermal matrix while maintaining flexible properties essential for skin applications. In vitro, collagen hydrogel scaffolds retained their open porous architecture and viably sustained human fibroblasts and murine mesenchymal stem cells and endothelial cells. In vivo, hydrogel-treated murine excisional wounds demonstrated improved wound closure, which was associated with increased recruitment of stromal cells and formation of vascularized granulation tissue. In conclusion, salt-induced phase inversion techniques can be used to create modifiable pullulan-collagen composite dermal scaffolds that augment early wound healing. These novel biomatrices can potentially serve as a structured delivery template for cells and biomolecules in regenerative skin applications.
需要新的皮肤再生策略来解决由皮肤创伤和疾病引起的重大医疗负担。在这项研究中，使用盐诱导的相转化技术制备了普鲁兰-胶原复合水凝胶基质，从而为皮肤工程构建了具有结构但柔软的支架。盐结晶诱导了相互连接的孔形成，并且胶原浓度的修饰允许调节支架的孔径。水凝胶结构再现了人真皮基质的网状分布，同时保持了皮肤应用所必需的柔性。在体外，胶原水凝胶支架保留了其开放多孔结构，并能有效地维持人成纤维细胞和小鼠间充质干细胞和内皮细胞的存活。在体内，水凝胶处理的小鼠切创伤口显示出改善的伤口闭合，这与基质细胞的募集增加和血管化肉芽组织的形成有关。总之，盐诱导的相转化技术可用于创建可修饰的普鲁兰-胶原复合真皮支架，可增强早期伤口愈合。这些新型生物基质有可能作为再生皮肤应用中细胞和生物分子的结构化递药模板。
