Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, California, USA.
TauTona Group, Redwood City, California, USA.
Wound Repair Regen. 2022 May;30(3):397-408. doi: 10.1111/wrr.13012. Epub 2022 Apr 18.
Biological scaffolds such as hydrogels provide an ideal, physio-mimetic of native extracellular matrix (ECM) that can improve wound healing outcomes after cutaneous injury. While most studies have focused on the benefits of hydrogels in accelerating wound healing, there are minimal data directly comparing different hydrogel material compositions. In this study, we utilized a splinted excisional wound model that recapitulates human-like wound healing in mice and treated wounds with three different collagen hydrogel dressings. We assessed the feasibility of applying each dressing and performed histologic and histopathologic analysis on the explanted scar tissues to assess variations in collagen architecture and alignment, as well as the tissue response. Our data indicate that the material properties of hydrogel dressings can significantly influence healing time, cellular response, and resulting architecture of healed scars. Specifically, our pullulan-collagen hydrogel dressing accelerated wound closure and promoted healed tissue with less dense, more randomly aligned, and shorter collagen fibres. Further understanding of how hydrogel properties affect the healing and resulting scar architecture of wounds may lead to novel insights and further optimization of the material properties of wound dressings.
生物支架如水凝胶提供了一个理想的、类似生理的天然细胞外基质（ECM），可以改善皮肤损伤后的伤口愈合效果。虽然大多数研究都集中在水凝胶在加速伤口愈合方面的益处，但很少有数据直接比较不同水凝胶材料组成。在这项研究中，我们利用了一种夹板式切除术模型，该模型在小鼠中重现了类似人类的伤口愈合，并使用三种不同的胶原水凝胶敷料处理伤口。我们评估了每种敷料的应用可行性，并对取出的瘢痕组织进行组织学和组织病理学分析，以评估胶原结构和排列的变化以及组织反应。我们的数据表明，水凝胶敷料的材料特性可以显著影响愈合时间、细胞反应和愈合后瘢痕的结构。具体来说，我们的普鲁兰-胶原水凝胶敷料加速了伤口闭合，并促进了愈合组织，其胶原纤维密度更低、排列更随机且更短。进一步了解水凝胶特性如何影响伤口的愈合和最终瘢痕结构，可能会为伤口敷料的材料特性带来新的见解和进一步优化。
