Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4.
J Microbiol Methods. 2010 Dec;83(3):296-301. doi: 10.1016/j.mimet.2010.09.020. Epub 2010 Oct 12.
The human gastrointestinal tract hosts a complex community of microorganisms that grow as biofilms on the intestinal mucosa. These bacterial communities are not well characterized, although they are known to play an important role in human health. This study aimed to develop a model for culturing biofilms (surface-adherent communities) of intestinal microbiota. The model utilizes adherent mucosal bacteria recovered from colonic biopsies to create multi-species biofilms. Culture on selective media and confocal microscopy indicated the biofilms were composed of a diverse community of bacteria. Molecular analyses confirmed that several phyla were represented in the model, and demonstrated stability of the community over 96 h when cultured in the device. This model is novel in its use of a multi-species community of mucosal bacteria grown in a biofilm mode of growth.
人类胃肠道中栖息着复杂的微生物群落，它们在肠黏膜上形成生物膜。这些细菌群落尚未得到充分描述，尽管已知它们在人类健康中发挥着重要作用。本研究旨在开发一种培养肠道微生物群落生物膜（表面附着群落）的模型。该模型利用从结肠活检中回收的附着黏膜细菌来创建多物种生物膜。选择性培养基培养和共聚焦显微镜分析表明，生物膜由多种细菌组成。分子分析证实，该模型中存在几个门，并且当在设备中培养时，在 96 小时内群落具有稳定性。该模型的新颖之处在于它使用了在生物膜生长模式下生长的多种黏膜细菌群落。
