使用填充柱生物膜反应器和体外恒化器模型模拟远端肠道黏膜和管腔群落。
Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model.
作者信息
McDonald Julie A K, Fuentes Susana, Schroeter Kathleen, Heikamp-deJong Ineke, Khursigara Cezar M, de Vos Willem M, Allen-Vercoe Emma
机构信息
Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
出版信息
J Microbiol Methods. 2015 Jan;108:36-44. doi: 10.1016/j.mimet.2014.11.007. Epub 2014 Nov 20.
In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.
对人类肠道黏膜微生物群的体内研究通常仅限于终点分析，并且会受到肠道清洁程序的干扰。因此，我们使用生物膜反应器将模拟的黏膜环境纳入体外肠道恒化器模型。所形成的群落复杂、可重复、独特，并且代表了体内群落。
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