McDonald Julie A K, Fuentes Susana, Schroeter Kathleen, Heikamp-deJong Ineke, Khursigara Cezar M, de Vos Willem M, Allen-Vercoe Emma
Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada.
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
J Microbiol Methods. 2015 Jan;108:36-44. doi: 10.1016/j.mimet.2014.11.007. Epub 2014 Nov 20.
In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.
对人类肠道黏膜微生物群的体内研究通常仅限于终点分析，并且会受到肠道清洁程序的干扰。因此，我们使用生物膜反应器将模拟的黏膜环境纳入体外肠道恒化器模型。所形成的群落复杂、可重复、独特，并且代表了体内群落。
