用脱氧胆酸钠从感染细胞中提取的猿猴病毒40 DNA不再反映其体内超螺旋密度。
Simian virus 40 DNA extracted from infected cells with sodium deoxycholate no longer reflects its in vivo superhelix density.
作者信息
Germond J E
出版信息
J Virol. 1978 Jun;26(3):681-5. doi: 10.1128/JVI.26.3.681-685.1978.
Abstract
When simian virus 40 DNA is extracted from infected cells with low concentrations of sodium deoxycholate, which selectively extract non-encapsidated simian virus 40 DNA, the DNA has a lower average number of superhelical turns than the DNA extracted from purified viral particles. During extraction, a partial deproteinization of the DNA by a concentration of detergent that did not inactivate a nicking-closing activity led to the removal of some superhelical turns. The DNA extracted in this way no longer reflected its in vivo number of superhelical turns.
摘要
当用低浓度脱氧胆酸钠从受感染细胞中提取猴病毒40 DNA时，脱氧胆酸钠会选择性地提取未包裹的猴病毒40 DNA，此时提取出的DNA超螺旋圈数的平均数低于从纯化病毒颗粒中提取的DNA。在提取过程中，一定浓度的去污剂使DNA发生部分脱蛋白作用，但该去污剂并未使切口封闭活性失活，这种脱蛋白作用导致一些超螺旋圈被去除。以这种方式提取的DNA不再反映其在体内的超螺旋圈数。
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