Yonsei University, Seodaemungu, Seoul, South Korea.
Appl Microbiol Biotechnol. 2011 Feb;89(3):835-42. doi: 10.1007/s00253-010-2893-8. Epub 2010 Oct 5.
Due to the environmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. However, little is known about the microbial risk present in sludge and its stabilized products. This study microbiologically explored the effects of anaerobic lagoon fermentation (ALF) and autothermal thermophilic aerobic digestion (ATAD) on pathogen-related risk of raw swine manure by using culture-independent 16S rDNA cloning and sequencing methods. In raw swine manure, clones closely related to pathogens such as Dialister pneumosintes, Erysipelothrix rhusiopathiae, Succinivibrioan dextrinosolvens, and Schineria sp. were detected. Meanwhile, in the mesophilic ALF-treated swine manure, bacterial community clones closely related to pathogens such as Schineria sp. and Succinivibrio dextrinosolvens were still detected. Interestingly, the ATAD treatment resulted in no detection of clones closely related to pathogens in the stabilized thermophilic bacterial community, with the predominance of novel Clostridia class populations. These findings support the superiority of ATAD in selectively reducing potential human and animal pathogens compared to ALF, which is a typical manure stabilization method used in livestock farms.
由于与牲畜污泥处置相关的环境问题，许多强调污泥体积减少的稳定化研究已经进行。然而，对于污泥及其稳定化产物中存在的微生物风险知之甚少。本研究使用非培养 16S rDNA 克隆和测序方法，从微生物学角度探讨了厌氧塘发酵（ALF）和自热嗜热好氧消化（ATAD）对原猪粪中与病原体相关的风险的影响。在原猪粪中，检测到与病原体如 Dialister pneumosintes、Erysipelothrix rhusiopathiae、Succinivibrioan dextrinosolvens 和 Schineria sp. 密切相关的克隆。同时，在中温 ALF 处理的猪粪中，仍检测到与病原体如 Schineria sp. 和 Succinivibrio dextrinosolvens 密切相关的细菌群落克隆。有趣的是，ATAD 处理导致稳定的嗜热细菌群落中未检测到与病原体密切相关的克隆，而新型梭菌种群占主导地位。这些发现支持了 ATAD 相对于典型的用于牲畜场的粪便稳定化方法 ALF 在选择性减少潜在的人类和动物病原体方面的优越性。
