Ghimire Sudeep, Wongkuna Supapit, Scaria Joy
Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, United States of America.
PeerJ. 2020 Oct 5;8:e10071. doi: 10.7717/peerj.10071. eCollection 2020.
A Gram-positive, non-motile, rod-shaped facultative anaerobic bacterial strain SG502 was isolated from healthy human fecal samples in Brookings, SD, USA. The comparison of the 16S rRNA gene placed the strain within the family . Within this family, ATCC 14501, strain PG-426-CC-2, DSM 3991 and DSM 3987(=ATCC 25548) were its closest taxa with 95.28%, 94.17%, 93.25%, and 92.75% 16S rRNA sequence identities respectively. The strain SG502 placed itself close to in the 16S rRNA phylogeny. The members of genus within family was proposed to be reassigned to genus to resolve the misclassification of genus . Therefore, was also classified into this genus temporarily with the need to reclassify it in the future because of its difference in genomic properties. Similarly, genome sequencing of the strain and comparison with its 16S phylogenetic members and proposed members of the genus , SG502 warranted a separate genus even though its 16S rRNA similarity was >95% when comapred to . The strain was 71.8% similar at ANI, 19.8% [17.4-22.2%] at dDDH and 69.65% similar at AAI to its closest neighbor . The genome size was nearly 2,683,792 bp with 32.88 mol% G+C content, which is about half the size of genome and the G+C content revealed 10 mol% difference. Phenotypically, the optimal growth temperature and pH for the strain SG502 were 37 °C and 7.0 respectively. Acetate was the major short-chain fatty acid product of the strain when grown in BHI-M medium. The major cellular fatty acids produced were C 9c, Cand C. Thus, based on the polyphasic analysis, for the type strain SG502 (=DSM 107282= CCOS 1889), the name gen. nov., sp. nov., is proposed.
从美国南达科他州布鲁金斯市健康人粪便样本中分离出一株革兰氏阳性、无运动性、杆状兼性厌氧细菌菌株SG502。16S rRNA基因比较将该菌株置于该科内。在这个科中，ATCC 14501、菌株PG - 426 - CC - 2、DSM 3991和DSM 3987（=ATCC 25548）是其最接近的分类单元，16S rRNA序列同一性分别为95.28%、94.17%、93.25%和92.75%。在16S rRNA系统发育中，菌株SG502与……接近。为解决……属的错误分类问题，该科内……属的成员被提议重新归入……属。因此，由于其基因组特性存在差异，SG502也暂时归入该属，未来需要重新分类。同样，对该菌株进行全基因组测序，并与其16S系统发育成员以及提议的……属成员进行比较，尽管与……相比其16S rRNA相似性>95%，但SG502仍有理由成为一个独立的属。该菌株与最接近的邻居……在ANI上相似性为71.8%，在dDDH上为19.8%[17.4 - 22.2%]，在AAI上为69.65%。基因组大小约为2,683,792 bp，G + C含量为32.88 mol%，约为……基因组大小的一半，G + C含量相差10 mol%。表型上，菌株SG502的最适生长温度和pH分别为37°C和7.0。当在BHI - M培养基中生长时，乙酸是该菌株产生的主要短链脂肪酸产物。产生的主要细胞脂肪酸为C9c、C和C。因此，基于多相分析，对于模式菌株SG502（=DSM 107282 = CCOS 1889），提议命名为……新属，……新种。
