Sekiguchi M, Sakakibara K, Fujii G
Jpn J Exp Med. 1978 Feb;48(1):61-8.
A human gastric carcinoma cell line, KATO-II, and its subline, KATO-III, have been established in vitro from a pleural effusion of a 55-year-old male patient. Kato-II cells have been maintained using a culture medium containing human cord serum and KATO-III cells have been cultured with a medium containing fetal bovine serum. Both cell lines are morphologically quite similar; they grow in vitro floating free and have cytological features of signet ring cells. Population doubling times for KATO-II and KATO-III were 74 hours and 36 hours, repsectively. The modal chromosomal numbers of these cell lines fell in a tetraploid range. Heterotransplantation of KATO-II and KATO-III cells was successfully done in the cheek pouches of antithymocyte serum-treated hamsters. Microscopical appearance of the resultant tumors was consistent with poorly-differentiated adenocarcinoma comparable to the original tumor.
一株人胃癌细胞系KATO-II及其亚系KATO-III是从一名55岁男性患者的胸腔积液中体外建立的。Kato-II细胞使用含人脐带血清的培养基进行培养，而KATO-III细胞则用含胎牛血清的培养基培养。这两种细胞系在形态上非常相似；它们在体外自由漂浮生长，具有印戒细胞的细胞学特征。KATO-II和KATO-III的群体倍增时间分别为74小时和36小时。这些细胞系的众数染色体数处于四倍体范围内。KATO-II和KATO-III细胞在经抗胸腺细胞血清处理的仓鼠颊囊中成功进行了异种移植。所形成肿瘤的显微镜下表现与原始肿瘤相似，为低分化腺癌。
