Kitsuki H, Morisaki T, Katano M, Torisu M
Department of Surgery I, Kyushu University Faculty of Medicine, Fukuoka, Japan.
Hum Cell. 1994 Sep;7(3):145-50.
We have established a human colonic carcinoma cell line, designated KT-COLO-8, derived from cancerous ascites of a 64-year-old male patient who had mucinous adenocarcinoma of the transverse colon. The cells have been cultured with RPMI-1640 medium supplemented with 10% fetal bovine serum. They grew in vitro free-floating and formed sheet-like clusters as large as 2-3 mm in diameter. Population doubling time for the cells was 48 hours. The chromosome number ranged from 48 to 50. The Q-band analysis revealed abnormal karyotype with three marker chromosomes including 1p-, 4q- and 5q-. The cells produced and released high level of carcinoembryonic antigen (CEA) in the conditioned medium. Percentage of CEA positive cells was 98%. An aggregation assay revealed that 80% of the cells dissociated into single cells aggregated within 30 min. The self aggregation of the cells was completely inhibited only by anti-CEA monoclonal antibody, suggesting that the cluster formation of KT-COLO-8 cells was dependent on CEA molecules.
我们建立了一种人结肠癌细胞系，命名为KT - COLO - 8，它源自一名64岁男性患者的癌性腹水，该患者患有横结肠黏液腺癌。这些细胞在补充有10%胎牛血清的RPMI - 1640培养基中培养。它们在体外自由漂浮生长，并形成直径达2 - 3毫米的片状聚集体。细胞的群体倍增时间为48小时。染色体数目在48至50之间。Q带分析显示核型异常，有三条标记染色体，包括1p -、4q -和5q -。这些细胞在条件培养基中产生并释放高水平的癌胚抗原（CEA）。CEA阳性细胞的百分比为98%。一项聚集试验显示，80%解离成单细胞的细胞在30分钟内聚集。细胞的自我聚集仅被抗CEA单克隆抗体完全抑制，这表明KT - COLO - 8细胞的聚集体形成依赖于CEA分子。
