Cell Cycle and Cancer Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Genetics. 2010 Dec;186(4):1247-60. doi: 10.1534/genetics.110.123257. Epub 2010 Oct 5.
Slk19p is a member of the Cdc-14 early anaphase release (FEAR) pathway, a signaling network that is responsible for activation of the cell-cycle regulator Cdc14p in Saccharomyces cerevisiae. Disruption of the FEAR pathway results in defects in anaphase, including alterations in the assembly and behavior of the anaphase spindle. Many phenotypes of slk19Δ mutants are consistent with a loss of FEAR signaling, but other phenotypes suggest that Slk19p may have FEAR-independent roles in modulating the behavior of microtubules in anaphase. Here, a series of SLK19 in-frame deletion mutations were used to test whether Slk19p has distinct roles in anaphase that can be ascribed to specific regions of the protein. Separation-of-function alleles were identified that are defective for either FEAR signaling or aspects of anaphase spindle function. The data suggest that in early anaphase one region of Slk19p is essential for FEAR signaling, while later in anaphase another region is critical for maintaining the coordination between spindle elongation and the growth of interpolar microtubules.
Slk19p 是 Cdc-14 早后期释放（FEAR）途径的成员，该途径是一个信号网络，负责激活酿酒酵母中的细胞周期调节剂 Cdc14p。FEAR 途径的破坏会导致后期缺陷，包括后期纺锤体的组装和行为改变。slk19Δ 突变体的许多表型与 FEAR 信号的丧失一致，但其他表型表明 Slk19p 可能在调节后期微管行为方面具有 FEAR 非依赖性作用。在这里，使用一系列 SLK19 框内缺失突变来测试 Slk19p 是否在后期间期具有可以归因于蛋白质特定区域的不同作用。分离功能等位基因被鉴定为 FEAR 信号或后期纺锤体功能方面的缺陷。数据表明，在早期后期，Slk19p 的一个区域对于 FEAR 信号是必需的，而在后期的后期，另一个区域对于维持纺锤体伸长和极间微管生长之间的协调至关重要。
