利用聚合酶链反应从壳膜样品中提取 DNA 对德尼兹利鸡进行雏鸡性别鉴定。

Sexing of newly-hatched chicks using DNA isolated from chorio-allantoic membrane samples by polymerase chain reaction in Denizli chicken.

Adnan Menderes Universitesi Veteriner Fakultesi, Zootekni.

Br Poult Sci. 2010 Aug;51(4):525-9. doi: 10.1080/00071668.2010.502521.

Abstract

The aim of the present study was to determine the sex of newly-hatched chicks of Denizli chicken, a local Turkish breed, by polymerase chain reaction (PCR) using DNA extracted from the chorioallantoic membrane (CAM). 2. Fertilised eggs were incubated individually and a total of 20 CAM samples were collected following the hatching process. DNA was isolated from the CAM samples and PCR was performed using W-repeat (W) and 18 S ribosomal gene (R) primers. 3. Screening of the PCR products by agarose gel electrophoresis revealed that males have a single band (256 bp) and females have an extra second band (415 bp) as expected. 4. The present study describes a reliable, rapid, and simple multiplex PCR protocol that can be put into use to sex local breeds of chicken in which phenotypic sexing is impossible, using DNA isolated from the CAM that is discarded and remains attached to the egg shell following the hatching process.

摘要

本研究旨在通过聚合酶链反应（PCR），利用从绒毛尿囊膜（CAM）中提取的 DNA，确定德尼兹利鸡（一种土耳其本地品种）刚孵化小鸡的性别。
将受精卵单独孵化，在孵化过程后共收集了 20 个 CAM 样本。从 CAM 样本中提取 DNA，然后使用 W-重复（W）和 18S 核糖体基因（R）引物进行 PCR。
通过琼脂糖凝胶电泳对 PCR 产物进行筛选，结果表明雄性有一条单带（256bp），而雌性有一条额外的第二条带（415bp），这与预期相符。
本研究描述了一种可靠、快速且简单的多重 PCR 方案，可用于对表型性别鉴定不可能的本地鸡种进行性别鉴定，使用从 CAM 中分离的 DNA，CAM 是在孵化过程后附着在蛋壳上的废弃组织。