反相高效液相色谱法同时测定凤仙叶中的三种萘醌

Simultaneous determination of three naphthoquinones in the leaves of Impatiens balsamina L. by reversed-phase high-performance liquid chromatography.

机构信息

Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.

出版信息

Phytochem Anal. 2010 Sep-Oct;21(5):444-50. doi: 10.1002/pca.1216.

DOI:10.1002/pca.1216

PMID:20931623

Abstract

INTRODUCTION

Naphthoquinones; lawsone (1), lawsone methyl ether (2) and methylene-3,3'-bilawsone (3) are the main active compounds of Impatiens balsamina leaves.

OBJECTIVE

To develop and validate an HPLC method for simultaneous quantitative determination of 1-3 in I. balsamina leaf extracts.

METHODOLOGY

The method utilised a Supelco® C(18) column (5 µm, 4.6 × 150 mm) at 25°C with the mixture of 2% aqueous acetic acid : methanol (gradient elution as follows: 0-10 min, 25 : 75; 10-20 min, 32 : 68; 20-35 min, 55 : 45) as the mobile phase at a flow-rate of 1 mL/min, and UV detection at 280 nm. The parameters of linearity, repeatability, reproducibility, accuracy specificity and sensitivity of the method were evaluated.

RESULTS

The recovery of the method was 96-101% and linearity (r(2) ≥ 0.9995) was obtained for all naphthoquinones. A high degree of specificity, as well as repeatability and reproducibility (RSD less than 5%), were also achieved.

摘要

简介

萘醌；指甲花醌（1）、指甲花醌甲醚（2）和亚甲基-3,3'-联萘醌（3）是凤仙花叶片的主要活性化合物。

目的

建立并验证一种同时定量测定凤仙花叶提取物中 1-3 的 HPLC 方法。

方法

该方法采用 Supelco® C(18)柱（5μm，4.6×150mm），在 25°C 下，以 2%的水乙酸：甲醇（梯度洗脱如下：0-10 分钟，25:75；10-20 分钟，32:68；20-35 分钟，55:45）作为流动相，流速为 1mL/min，UV 检测波长为 280nm。对该方法的线性、重复性、重现性、准确性、特异性和灵敏度参数进行了评价。