Department of Science & Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory (CSIR), Dr. K.S. Krishnan Marg, New Delhi 110012, India.
J Biotechnol. 2010 Nov;150(3):357-65. doi: 10.1016/j.jbiotec.2010.09.935. Epub 2010 Oct 8.
A sequence-specific electrochemical sexually transmitted disease (STD) sensor based on self-assembled monolayer of thiolated DNA probe specific to target opa gene for detection of Gonorrhoea, a sexually transmitted disease has been fabricated. 6-Mercapto-1-hexanol (MCH) has been used as a blocking agent to facilitate oligos "stand" up at the surface, a configuration favoring subsequent DNA hybridization and to repel non-specific adsorption of undesired DNA. The results of differential pulse voltammetric studies of this STD sensor reveal low detection limit (1.0 × 10(-18)M) and a wide dynamic range (from 1.0 × 10(-6)M to 0.5 × 10(-18)M) arising due to the stable hybridization using methylene blue as an electro-active DNA hybridization indicator. The experimental results with genomic DNA, clinical patient sample of Neisseria gonorrhoeae, culture of non-N. gonorrhoeae Neisseria species (NgNS) and gram negative bacteria indicate that the fabricated sensor is specific to this STD.
已制备了一种基于针对靶标 opa 基因的硫醇化 DNA 探针的自组装单层的序列特异性电化学生殖道疾病（STD）传感器，用于检测淋病，一种性传播疾病。使用 6-巯基-1-己醇（MCH）作为阻断剂，以促进寡核苷酸在表面“站立”，这种构象有利于随后的 DNA 杂交，并排斥不需要的 DNA 的非特异性吸附。该 STD 传感器的差分脉冲伏安研究结果表明，由于使用亚甲基蓝作为电活性 DNA 杂交指示剂进行稳定杂交，因此具有低检测限（1.0×10^（-18）M）和宽动态范围（从 1.0×10^（-6）M 到 0.5×10^（-18）M）。用基因组 DNA、淋病奈瑟氏球菌的临床患者样本、非淋病奈瑟氏菌属（NgNS）和革兰氏阴性细菌的培养物进行的实验结果表明，所制备的传感器对该 STD 具有特异性。
