Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore.
J Colloid Interface Sci. 2011 Jan 1;353(1):143-8. doi: 10.1016/j.jcis.2010.09.029. Epub 2010 Sep 17.
In this paper, we report a method of printing uniform protein lines on glass slides by using UV-treated flat PDMS stamps. Unlike traditional microcontact printing (μCP) which requires microstructured PDMS stamps, this μCP method only requires a flat PDMS stamp, an UV lamp and a number of straight needles. Our results show that lines of bovine serum albumin (BSA), immunoglobin (IgG), anti-biotin, anti-human IgG and anti-mouse IgG can be printed evenly on glass slides by using this μCP method. We also demonstrate that the printed protein lines are suitable for applications such as microfluidic immunoassays.
本文报道了一种在玻璃载片上打印均匀蛋白线的方法，该方法使用经紫外光处理的平整 PDMS 印章。与传统的微接触印刷（μCP）需要微结构的 PDMS 印章不同，这种 μCP 方法仅需要一个平整 PDMS 印章、一个紫外光灯和一些直针。我们的结果表明，使用这种 μCP 方法可以在玻璃载片上均匀地打印出牛血清白蛋白（BSA）、免疫球蛋白（IgG）、抗生物素、抗人 IgG 和抗鼠 IgG 等蛋白线。我们还证明了打印的蛋白线适用于微流控免疫分析等应用。
