School of Civil and Environmental Engineering, Cornell University, Ithaca, NY 14853, USA.
Bioresour Technol. 2011 Jan;102(2):1389-98. doi: 10.1016/j.biortech.2010.09.035. Epub 2010 Sep 17.
The objective of this research was to measure the effects of different cellulase and hemicellulase mixtures on fermentable sugar production from two different perennial biomasses--switchgrass and a low-impact, high-diversity prairie biomass mixture (LIHD). Each was subjected to NaOH pretreatment, followed by hydrolysis with a commercial cellulase and β-glucosidase mixture [CB] supplemented with either of two hemicellulases. For both biomasses, there was little gain in sugar yield when using CB alone beyond 20-25 mg/g TS; further gain in yield was possible only through hemicellulase supplementation. An equation that modeled CB and hemicellulase effects as occurring independently fit the data reasonably well, except at the lowest of cellulase loadings with hemicellulase, where synergistic interactions were evident. Examination of the marginal effectiveness of enzyme loadings (incremental grams sugar per incremental mg enzyme) over a broad range of loadings suggests that there is no need to customize enzymatic hydrolysis for NaOH-pretreated switchgrass and LIHD.
本研究的目的是测量不同纤维素酶和半纤维素酶混合物对两种不同多年生生物质——柳枝稷和低影响、高多样性草原生物质混合物（LIHD）的发酵糖生产的影响。每种生物质都经过氢氧化钠预处理，然后用商业纤维素酶和β-葡萄糖苷酶混合物[CB]进行水解，并用两种半纤维素酶中的一种进行补充。对于这两种生物质，单独使用 CB 进行水解，在 20-25mg/gTS 之外，糖产量几乎没有增加；只有通过半纤维素酶的补充，才能进一步提高产量。一个将 CB 和半纤维素酶的作用建模为独立发生的方程很好地拟合了数据，除了在最低纤维素酶负荷与半纤维素酶的情况下，协同作用明显。对酶负荷的边际效应（每增加 1mg 酶可增加的糖克数）在广泛的负荷范围内进行考察表明，对于氢氧化钠预处理的柳枝稷和 LIHD，不需要定制酶水解。
