Givens R S, Jencen D A, Riley C M, Stobaugh J F, Chokshi H, Hanaoka N
Center for Bioanalytical Research, University of Kansas, Lawrence 66045.
J Pharm Biomed Anal. 1990;8(6):477-91. doi: 10.1016/0731-7085(90)80057-v.
The generation of light from the oxidation of oxalic esters with hydrogen peroxide has been applied to the detection of luminescent materials. In order to improve the efficiency of this method, which is less than 0.1%, and to enhance the selectivity for target analytes, an in-depth investigation of the oxalate ester-hydrogen peroxide reaction has been conducted. A kinetic model has been developed based on the effects of catalysts, reagents and reaction conditions for maximum light production. Application of the model to liquid chromatography through the "time-dependent emission window" concept affords a predictable maximum sensitivity for selected analytes. Application to the detection and quantitation of met- and leu-enkephalins which have been labelled with naphthalene-2,3-dicarboxyaldehyde/cyanide provides support for this methodology. Other bioanalytical applications are presented.
草酸酯与过氧化氢氧化反应产生的光已应用于发光材料的检测。为了提高该方法（效率低于0.1%）的效率并增强对目标分析物的选择性，已对草酸酯 - 过氧化氢反应进行了深入研究。基于催化剂、试剂和反应条件对最大光产量的影响建立了动力学模型。通过“时间相关发射窗口”概念将该模型应用于液相色谱，可为选定的分析物提供可预测的最大灵敏度。将其应用于用萘 - 2,3 - 二甲醛/氰化物标记的蛋氨酸脑啡肽和亮氨酸脑啡肽的检测和定量，为该方法提供了支持。还介绍了其他生物分析应用。
