Yang Fengzhen, Zhang Chao, Baeyens Willy R G, Zhang Xinrong
Department of Chemistry, Tsinghua University, 100084 Beijing, People's Republic of China.
J Pharm Biomed Anal. 2002 Oct 15;30(3):473-8. doi: 10.1016/s0731-7085(02)00096-1.
Strong chemiluminescence emission has been observed by mixing alkaline hydrolytic products of ethamsylate with Tween 80 in acidic rhodamine 6G solution. This phenomenon has been utilized to design a flow-injection chemiluminescence method for the determination of ethamsylate in a pharmaceutical preparation. Under the optimum conditions, the proposed procedure has a linear range between 0.05 and 2.0 microg ml(-1), with a detection limit of 0.02 microg ml(-1) for ethamsylate. The method was applied to the determination of ethamsylate in pharmaceutical preparations. The possible mechanism of this chemiluminescence reaction was proposed.
