Department of Histology, Embryology, and Applied Biology, Division of Prenatal Medicine, University of Bologna, Bologna, Italy.
Am J Obstet Gynecol. 2010 Dec;203(6):575.e1-7. doi: 10.1016/j.ajog.2010.07.043.
The purpose of this study was to determine whether the combined distribution of a panel of cellular messenger RNA markers can detect preeclampsia long before onset.
We compared blood at 10-14 weeks from 11 women who ultimately experienced preeclampsia with 88 matched control subjects. After multiples of the median conversion of all the markers, logistic regression was used to calculate the risk of the development of preeclampsia.
Higher multiples of the median values than expected were found for endoglin, fms-related tyrosine kinase 1, and transforming growth factor-β1. Lower multiples of the median values were found for placental growth factor and placental protein 13. Endoglin fms-related tyrosine kinase 1 and transforming growth factor-β1 had the best discriminant power. Messenger RNA species provided independent contributions to the prediction of preeclampsia. In fact, 11 women with preeclampsia scored a median risk of 50% of experiencing preeclampsia. Control subjects scored a median risk of preeclampsia of 0.18%. The detection rate at a 5% false positive rate was 72.3%.
The messenger RNA dosage in maternal blood would be a useful method for the calculation of the risk of the development of preeclampsia.
本研究旨在确定一组细胞信使 RNA 标志物的联合分布是否可以在先兆子痫发病前很久就检测到该病。
我们比较了 11 名最终发生先兆子痫的女性和 88 名匹配对照者在 10-14 周时的血液。对所有标志物的中位数倍数进行转换后，采用逻辑回归计算发生先兆子痫的风险。
发现内皮糖蛋白、fms 相关酪氨酸激酶 1 和转化生长因子-β1 的中位数倍数高于预期。胎盘生长因子和胎盘蛋白 13 的中位数倍数较低。内皮糖蛋白、fms 相关酪氨酸激酶 1 和转化生长因子-β1 具有最佳的判别能力。信使 RNA 种类对先兆子痫的预测有独立的贡献。事实上，11 名先兆子痫患者的中位数风险为 50%发生先兆子痫。对照组的先兆子痫中位数风险为 0.18%。在假阳性率为 5%时的检出率为 72.3%。
母体血液中的信使 RNA 含量是计算先兆子痫发生风险的一种有用方法。
