Synthesis of monodispersed molecularly imprinted polymer particles for high-performance liquid chromatographic separation of cholesterol using templating polymerization in porous silica gel bound with cholesterol molecules on its surface.

Monodispersed molecularly imprinted polymer particles selective for cholesterol were prepared by the copolymerization of styrene and divinylbenzene in the presence of template silica gel particles (particle size: 5 μm; pore size: 10 nm) functionalized with cholesterol on the surface, followed by dissolution of the cholesterol-bonded silica gel with a NaOH aqueous solution. Transmission and scanning electron micrographs of the molecularly imprinted polymer (MIP) particles revealed good monodispersity and porous structure. The MIP particles were packed into a high performance liquid chromatographic column, and its recognition ability of cholesterol was evaluated using cholesterol, cholesterol esters and fatty acid methyl esters by comparison with the non-imprinted polymer (NIP) particles prepared from styrene and divinylbenzene without cholesterol. The MIP particles showed a high affinity for cholesterol and cholesterol esters (K(MIP)'/K(NIP)' > 5.7).