Department of Chemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
J Chromatogr A. 2010 Nov 12;1217(46):7249-54. doi: 10.1016/j.chroma.2010.09.041. Epub 2010 Sep 19.
Monodispersed molecularly imprinted polymer particles selective for cholesterol were prepared by the copolymerization of styrene and divinylbenzene in the presence of template silica gel particles (particle size: 5 μm; pore size: 10 nm) functionalized with cholesterol on the surface, followed by dissolution of the cholesterol-bonded silica gel with a NaOH aqueous solution. Transmission and scanning electron micrographs of the molecularly imprinted polymer (MIP) particles revealed good monodispersity and porous structure. The MIP particles were packed into a high performance liquid chromatographic column, and its recognition ability of cholesterol was evaluated using cholesterol, cholesterol esters and fatty acid methyl esters by comparison with the non-imprinted polymer (NIP) particles prepared from styrene and divinylbenzene without cholesterol. The MIP particles showed a high affinity for cholesterol and cholesterol esters (K(MIP)'/K(NIP)' > 5.7).
通过在表面功能化有胆固醇的硅胶颗粒（粒径：5μm；孔径：10nm）存在的条件下，使苯乙烯和二乙烯基苯共聚，制备了对胆固醇具有选择专一性的单分散分子印迹聚合物颗粒，随后用 NaOH 水溶液溶解键合有胆固醇的硅胶。印迹聚合物（MIP）颗粒的透射和扫描电子显微照片显示出良好的单分散性和多孔结构。将 MIP 颗粒填充到高效液相色谱柱中，并使用胆固醇、胆固醇酯和脂肪酸甲酯与没有胆固醇的苯乙烯和二乙烯基苯制备的非印迹聚合物（NIP）颗粒进行比较，评估了其对胆固醇的识别能力。MIP 颗粒对胆固醇和胆固醇酯表现出高亲和力（K（MIP）' / K（NIP）' > 5.7）。
