Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany.
Hum Immunol. 2011 Jan;72(1):74-9. doi: 10.1016/j.humimm.2010.10.003. Epub 2010 Oct 30.
Human ficolin-2 (L-ficolin; FCN2) is a serum protein binding to sugar moieties of different human micro-pathogens forcing phagocytosis. Here, we investigate the clinical significance of FCN2 in African children with either mild or severe malaria (n = 130 and n = 108, respectively) from Gabon by analyzing three promoter SNPs (-986G>A, -602G>A, and -4A>G) and one single nucleotide polymorphisms (SNP) in exon 8 (+6424G>T) using quantitative TaqMan, real-time polymerase chain reaction (PCR). In addition, we measured the ficolin-2 plasma levels at two time points: on admission (t(0), acute disease) and 4 weeks after treatment (t(1), healthy phase). Comparison of ficolin-2 plasma levels shows that ficolin-2 concentration is highest during acute severe disease. In addition, we determined polymorphisms in the promoter and all coding regions of FCN2 in 40 Gabonese. Linkage disequilibrium data revealed polymorphic allelic combination patterns in the FCN2 promoter region; strong allelic combinations at -986 and -4, and -557 and -64 were found. No FCN2 promoter haplotypes were significantly distributed between mild and severe cases.
人纤维胶凝蛋白-2（L-纤维胶凝蛋白；FCN2）是一种血清蛋白，可与不同人类微生物病原体的糖基结合，从而强制吞噬作用。在这里，我们通过分析三个启动子 SNP（-986G>A、-602G>A 和 -4A>G）和一个外显子 8 中的一个单核苷酸多态性（SNP）（+6424G>T），来研究加蓬的轻度和重度疟疾儿童（分别为 130 例和 108 例）中 FCN2 的临床意义，使用定量 TaqMan、实时聚合酶链反应（PCR）。此外，我们在两个时间点测量了纤维胶凝蛋白-2 的血浆水平：入院时（t（0），急性疾病）和治疗后 4 周（t（1），健康期）。纤维胶凝蛋白-2 血浆水平的比较表明，纤维胶凝蛋白-2 浓度在急性重度疾病期间最高。此外，我们在 40 名加蓬人中确定了 FCN2 启动子和所有编码区的多态性。连锁不平衡数据显示 FCN2 启动子区域存在多态等位基因组合模式；在-986 和-4 以及-557 和-64 处发现了强等位基因组合。轻度和重度病例之间没有明显分布 FCN2 启动子单倍型。
