离心式微流控系统用于初级扩增和二级实时 PCR。
Centrifugal microfluidic system for primary amplification and secondary real-time PCR.
机构信息
Laboratory for MEMS Applications, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, 79110, Freiburg, Germany.
出版信息
Lab Chip. 2010 Dec 7;10(23):3210-2. doi: 10.1039/c0lc00161a. Epub 2010 Oct 11.
Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.
预扩增是许多聚合酶链反应（PCR）协议的基础，但由于处理高拷贝 DNA 样本，存在严重的污染风险。因此，我们开发了一种包含预存储试剂的独立离心微流控系统；它可以在修改后的商用热循环仪中进行自动等分和实时 PCR 之前，对特定 DNA 序列进行预扩增。
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