Amasia Mary, Madou Marc
Department of Chemical Engineering & Materials Science, University of California, Irvine, CA, USA.
Bioanalysis. 2010 Oct;2(10):1701-10. doi: 10.4155/bio.10.140.
Microfluidic-based systems are ideal for handling small microliter volumes of samples and reagents, but 'real-world' or clinical samples for bioanalysis are often on the milliliter scale. We aimed to develop and validate a large-volume centrifugal or compact disc-based device for blood plasma separation, capable of processing 2 ml undiluted blood samples.
This automated blood sample preparation device was shown to yield high purity plasma in less than half the time of commercial plasma preparation tubes, while enabling integration with downstream analysis and detection steps.
This article draws upon a novel large-volume device to further illustrate the challenges in combining microfluidics structures with large-volume samples and the implications for sample-driven microfluidics systems.
基于微流控的系统非常适合处理微升体积的少量样品和试剂，但用于生物分析的“实际”或临床样品通常为毫升规模。我们旨在开发并验证一种用于血浆分离的大容量离心式或基于光盘的设备，该设备能够处理2毫升未稀释的血液样本。
这种自动化血液样本制备设备在不到市售血浆制备管一半的时间内就能产生高纯度血浆，同时还能与下游分析和检测步骤集成。
本文利用一种新型大容量设备，进一步说明了将微流控结构与大容量样品相结合时面临的挑战以及对样品驱动的微流控系统的影响。
