Center for Molecular and Biomolecular Imaging, 2220 French Family Science Center, Duke University, Chemistry Department, Durham, North Carolina 27708-0346, USA.
Magn Reson Med. 2011 Feb;65(2):313-9. doi: 10.1002/mrm.22622. Epub 2010 Oct 11.
Brown adipose tissue and white adipose tissue depots are noninvasively characterized in vitro and in vivo in healthy and obese mice using intermolecular zero-quantum coherence transitions between lipid and water spins. Intermolecular zero-quantum coherences enable selective detection of spatial correlation between water and lipid spins and thereby the hydration of fatty deposits with subvoxel resolution. At about a 100 mm distance scale, the major observed peaks are between water, methylene protons at 1.3 ppm, and olefinic protons at 5.3 ppm. Our in vitro results show that the methylene-olefinic intermolecular zero-quantum coherence signal is strong both in brown and white adipose tissues, but that the water-methylene intermolecular zero-quantum coherence signal is characteristic only of brown adipose tissue. In vivo, the ratio of these peaks is substantially higher in lean or young mice than in old or obese mice.
棕色脂肪组织和白色脂肪组织在健康和肥胖小鼠中可通过脂质和水质子间的分子间零量子相干跃迁进行非侵入式的体外和体内特征描述。分子间零量子相干使水和脂质自旋之间的空间相关性的选择性检测成为可能，从而以亚体素分辨率对脂肪沉积进行水合作用。在大约 100 毫米的距离尺度上，主要观察到的峰在水中、1.3 ppm 处的亚甲基质子和 5.3 ppm 处的烯烃质子之间。我们的体外结果表明，棕色和白色脂肪组织中均具有较强的亚甲基-烯烃分子间零量子相干信号，但仅在棕色脂肪组织中具有水质子-亚甲基分子间零量子相干信号。在体内，与瘦或年轻的小鼠相比，这些峰的比值在年老或肥胖的小鼠中要高得多。
