Bacon K B, Camp R D
Institute of Dermatology, United Medical School, Guy's Hospital, London, England.
Prostaglandins. 1990 Dec;40(6):603-14. doi: 10.1016/0090-6980(90)90005-g.
In view of the evidence that lymphocyte infiltrates are a constant feature of the skin lesions of psoriasis and the demonstration that certain hydroxylated metabolites of arachidonic acid are present in lesional psoriatic skin and possess lymphocyte chemoattractant properties, lipid extracts of samples from lesional and normal skin were assayed to determine which are the predominant lipid lymphocyte chemoattractants in psoriasis. Dilution-related lymphocyte chemoattractant activity was found in lipid extracts of stratum corneum samples from psoriatic lesions, but not in similar extracts of stratum corneum from the heels of normal volunteers, whereas aqueous extracts of the samples from both sources contained equivalent amounts of this activity. Subsequent purification of lesional stratum corneum lipid extracts by straight and reversed phase high performance liquid chromatography (HPLC) revealed the presence of at least two different lipid chemoattractants, one major component being identified as 12-(R)-hydroxy-5,8,10,14-eicosatetraenoic acid (12[R]-HETE) by its biological and chromatographic properties. These compounds may play a role in the pathogenesis of the lymphocyte infiltrates in psoriatic lesions.
鉴于淋巴细胞浸润是银屑病皮肤病变的一个持续特征，且有证据表明花生四烯酸的某些羟基化代谢产物存在于银屑病病变皮肤中并具有淋巴细胞趋化特性，因此对病变皮肤和正常皮肤样本的脂质提取物进行了检测，以确定银屑病中主要的脂质淋巴细胞趋化剂。在银屑病病变角质层样本的脂质提取物中发现了与稀释相关的淋巴细胞趋化活性，但在正常志愿者足跟角质层的类似提取物中未发现，而来自这两个来源的样本的水提取物中这种活性含量相当。随后通过正相和反相高效液相色谱（HPLC）对病变角质层脂质提取物进行纯化，结果显示至少存在两种不同的脂质趋化剂，其中一种主要成分根据其生物学和色谱特性被鉴定为12-(R)-羟基-5,8,10,14-二十碳四烯酸（12[R]-HETE）。这些化合物可能在银屑病病变中淋巴细胞浸润的发病机制中起作用。
