光照强度会影响烟草原生质体中转基因的 RNA 沉默。
Light intensity affects RNA silencing of a transgene in Nicotiana benthamiana plants.
机构信息
Department of Biology, University of Crete, PO Box 2208, GR-71409 Heraklion, Crete, Greece.
出版信息
BMC Plant Biol. 2010 Oct 12;10:220. doi: 10.1186/1471-2229-10-220.
BACKGROUND
Expression of exogenous sequences in plants is often suppressed through one of the earliest described RNA silencing pathways, sense post-transcriptional gene silencing (S-PTGS). This type of suppression has made significant contributions to our knowledge of the biology of RNA silencing pathways and has important consequences in plant transgenesis applications. Although significant progress has been made in recent years, factors affecting the stability of transgene expression are still not well understood. It has been shown before that the efficiency of RNA silencing in plants is influenced by various environmental factors.
RESULTS
Here we report that a major environmental factor, light intensity, significantly affects the induction and systemic spread of S-PTGS. Moreover, we show that photoadaptation to high or low light intensity conditions differentially affects mRNA levels of major components of the RNA silencing machinery.
CONCLUSIONS
Light intensity is one of the previously unknown factors that affect transgene stability at the post-transcriptional level. Our findings demonstrate an example of how environmental conditions could affect RNA silencing.
背景
外源序列在植物中的表达常常受到最早描述的 RNA 沉默途径之一的抑制，即 sense 转录后基因沉默（S-PTGS）。这种类型的抑制对我们了解 RNA 沉默途径的生物学做出了重要贡献，并对植物转基因应用具有重要意义。尽管近年来取得了重大进展，但影响转基因表达稳定性的因素仍未得到很好的理解。以前已经表明，植物中 RNA 沉默的效率受到各种环境因素的影响。
结果
在这里，我们报告了一个主要的环境因素，光照强度，显著影响 S-PTGS 的诱导和系统传播。此外，我们还表明，对高光或低光强度条件的光适应会对 RNA 沉默机制的主要成分的 mRNA 水平产生差异影响。
结论
光照强度是影响转录后水平转基因稳定性的未知因素之一。我们的发现证明了环境条件如何影响 RNA 沉默的一个例子。
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