Department of Obstetrics and Gynecology, Inselspital, Bern University Hospital and University of Bern, Bern, Switzerland.
Reprod Sci. 2010 Nov;17(11):1006-15. doi: 10.1177/1933719110377471.
The objective of the study was to determine the feasibility of generating a biodegradable, stem cell-loaded osteogenic composite graft from human placenta. Initially, a scaffold from human chorion membrane was produced. Human placenta mesenchymal stem cells (MSCs) derived from either first-trimester chorionic villi or term chorion membrane were differentiated osteogenically on this scaffold. Outgrowth, adherence, and osteogenic differentiation of cells were assessed by immunohistochemistry (IHC), scanning electron microscopy, protein expression, and real-time polymerase chain reaction (RT-PCR). Our results showed that a cell-free extracellular matrix scaffold can be generated from human chorion. Seeded MSCs densely adhered to that scaffold and were osteogenically differentiated. Calcium and alkaline phosphatase were detected in the cell-scaffold constructs as a proof of mineralization and findings were confirmed by IHC and RT-PCR results. This study shows for the first time that generation of an osteogenic composite graft using placental tissue is feasible. It might allow therapeutic application of autologous or allogeneic grafts in congenital skeletal defects by means of a composite graft.
本研究旨在确定从人胎盘制备可生物降解、负载干细胞的成骨复合移植物的可行性。首先，制备了人绒毛膜的支架。从早期绒毛或足月绒毛膜分离的人胎盘间充质干细胞（MSCs）在该支架上向成骨细胞分化。通过免疫组织化学（IHC）、扫描电子显微镜、蛋白质表达和实时聚合酶链反应（RT-PCR）评估细胞的生长、黏附和成骨分化。结果表明，可从人绒毛膜制备无细胞的细胞外基质支架。接种的 MSCs 密集黏附于该支架并向成骨细胞分化。细胞-支架构建体中检测到钙和碱性磷酸酶，以证明矿化，免疫组织化学和 RT-PCR 结果证实了这一点。本研究首次表明，使用胎盘组织制备成骨复合移植物是可行的。它可能通过复合移植物为先天性骨骼缺陷提供自体或同种异体移植物的治疗应用。
