Departamento de Ciência de Alimentos, Universidade Estadual de Campinas, SP, Brazil.
Fungal Biol. 2010 Oct;114(10):863-72. doi: 10.1016/j.funbio.2010.08.003. Epub 2010 Aug 13.
Studies of laccases from marine-derived fungi are limited. In the present work, putative laccase genes from three marine-derived basidiomycetes and their laccase activities were evaluated. High amounts of laccase were produced by the fungal strains Marasmiellus sp. CBMAI 1062 (971.2UL⁻¹) and Peniophora sp. CBMAI 1063 (709.03UL⁻¹) when grown for 21d at 28°C in MA2ASW medium prepared with artificial seawater. Marine-derived basidiomycetes produced multiple distinct laccase sequences of about 200bp with 73-90% similarity to terrestrial basidiomycete laccases. Marasmiellus sp. CBMAI 1062 and Tinctoporellus sp. CBMAI 1061 showed the greatest laccase gene diversity with three and four distinct putative laccase sequences, respectively. This is the first report of laccase genes from marine-derived fungi, and our results revealed new putative laccases produced by three basidiomycetes.
海洋来源真菌漆酶的研究有限。本工作评估了三株海洋来源担子菌的假定漆酶基因及其漆酶活性。在人工海水配制的 MA2ASW 培养基中，于 28°C 下培养 21d 后，真菌菌株 Marasmiellus sp. CBMAI 1062（971.2UL⁻¹）和 Peniophora sp. CBMAI 1063（709.03UL⁻¹）产生了大量的漆酶（971.2UL⁻¹）。海洋来源担子菌产生了多个约 200bp 的不同漆酶序列，与陆生担子菌漆酶的相似性为 73-90%。Marasmiellus sp. CBMAI 1062 和 Tinctoporellus sp. CBMAI 1061 分别具有三个和四个不同的假定漆酶序列，显示出最大的漆酶基因多样性。这是海洋来源真菌漆酶基因的首次报道，我们的结果揭示了三种担子菌产生的新的假定漆酶。
