Department of Internal Medicine II, Faculty of Medicine in Pilsen, Charles University in Prague, University Hospital in Pilsen, Czech Republic.
Anticancer Res. 2010 Sep;30(9):3579-83.
MicroRNAs (miRNAs), which are endogenously expressed regulatory noncoding RNAs, have an altered expression in tumor tissues. MiRNAs regulate cancer-related processes such as cell growth and tissue differentiation, and therefore, might function as oncogenes or tumor-suppressor genes. The aim of our study was to assess the expression of mir-20a, let-7a, miR-15a and miR-16 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) tissue and to investigate the relation between the expression of miRNAs and the clinicopathological features of PCa.
The study group comprised 138 patients: 85 patients with BPH and 53 patients with PCa. The total RNA was isolated from the tissue specimen core and miRNA expressions were quantified using a real-time RT-PCR method (TaqMan MicroRNA Assays). U6snRNA was used for the normalization of the miRNA expression.
miR-20a expression was significantly higher in the group of patients with a Gleason score of 7-10 in comparison with the group of patients with a Gleason score of 0-6 (p=0.0082). We found no statistical differences in the miRNA expressions (mir-20a, let-7a, miR-15a and miR-16) in the PCa tissue samples in comparison with the BPH tissue samples.
Our result shows that the more dedifferentiated PCa cells have a higher expression of miR-20a and this supports the oncogenic role of miR-20a in PCa carcinogenesis. The evaluation of miRNA expression could yield new information about PCa pathogenesis.
微小 RNA（miRNA）是内源性表达的调节性非编码 RNA，在肿瘤组织中表达异常。miRNA 调节与癌症相关的过程，如细胞生长和组织分化，因此可能作为癌基因或肿瘤抑制基因发挥作用。我们的研究目的是评估 mir-20a、let-7a、miR-15a 和 miR-16 在前列腺癌（PCa）和良性前列腺增生（BPH）组织中的表达，并探讨 miRNA 的表达与 PCa 临床病理特征之间的关系。
研究组包括 138 名患者：85 名 BPH 患者和 53 名 PCa 患者。从组织标本核心中分离总 RNA，并使用实时 RT-PCR 方法（TaqMan MicroRNA 测定）定量 miRNA 表达。U6snRNA 用于 miRNA 表达的归一化。
与 Gleason 评分 0-6 组相比，Gleason 评分 7-10 组患者的 miR-20a 表达显著升高（p=0.0082）。我们发现 PCa 组织样本与 BPH 组织样本之间 miRNA 表达（mir-20a、let-7a、miR-15a 和 miR-16）没有统计学差异。
我们的结果表明，分化程度较低的 PCa 细胞 miR-20a 的表达较高，这支持 miR-20a 在 PCa 发生中的致癌作用。miRNA 表达的评估可能为 PCa 发病机制提供新的信息。
