1Graduate School of Dalian Medical University, Dalian 116044, PR China.
Anticancer Res. 2010 Sep;30(9):3593-8.
To investigate whether down-regulation of P-glycoprotein (P-gp) is correlated to resistance to cisplatin and VP-16 in four histopathological subtype cell lines of lung cancer (SK-MES-1, SPCA-1, NCI-H-460 and NCI-H-446).
After pretreatment with or without verapamil, the P-gp expression was examined by means of RT-PCR and immunofluorescence. Cell survival on treatment with cisplatin and VP-16 was determined by MTT assay.
The expression of P-gp was clearly inhibited by verapamil in all four cell lines. Following pretreatment with verapamil, NCI-H-446 was more sensitive to cisplatin, while SPCA-1, NCI-H-460 and NCI-H-446 were more sensitive to VP-16 compared to the control.
Down-regulation of P-gp is associated with intrinsic resistance to cisplatin in the NCI-H-446 and to VP-16 in SPCA-1, NCI-H-460 and NCI-H-446 cell lines. These findings indicate that down-regulation of P-gp may be helpful for the reversion of drug resistance in some lung cancer cell line subtypes.
研究四种肺癌组织病理亚型细胞系（SK-MES-1、SPCA-1、NCI-H-460 和 NCI-H-446）中 P-糖蛋白（P-gp）下调是否与顺铂和 VP-16 的耐药性相关。
用或不用维拉帕米预处理后，通过 RT-PCR 和免疫荧光法检测 P-gp 的表达。用 MTT 法测定细胞对顺铂和 VP-16 的存活情况。
维拉帕米明显抑制了所有四种细胞系中 P-gp 的表达。与对照组相比，经维拉帕米预处理后，NCI-H-446 对顺铂更敏感，而 SPCA-1、NCI-H-460 和 NCI-H-446 对 VP-16 更敏感。
P-gp 的下调与 NCI-H-446 对顺铂的固有耐药性以及 SPCA-1、NCI-H-460 和 NCI-H-446 对 VP-16 的耐药性相关。这些发现表明，下调 P-gp 可能有助于逆转某些肺癌细胞系亚型的耐药性。
