Street J M, Trafford D J, Makin H L
J Chromatogr. 1985 Oct 11;343(2):259-70. doi: 10.1016/s0378-4347(00)84596-x.
A new method for the extraction of bile acids from human plasma using acetonitrile precipitation of plasma protein and subsequent use of Bond-Elut C18 cartridges is described. After extraction the bile acids can be separated into three fractions: unconjugated, glycine-, and taurine-conjugated, using Sep-Pak SIL cartridges at 4 degrees C, eluting with ethanol--chloroform--water--glacial acetic acid mixtures. These extraction and fractionation procedures were evaluated in terms of recovery, reproducibility and resolution between the fractions. All these parameters were found to be satisfactory. Although the reproducibility of fractionation on Sep-Pak SIL cartridges was found to vary between batches, this did not give rise to significant difficulties. Plasmas from normals and patients with hepatobiliary disease were analysed by capillary gas-liquid chromatography after extraction and fractionation using the procedure described.
本文描述了一种从人血浆中提取胆汁酸的新方法，该方法利用乙腈沉淀血浆蛋白，随后使用Bond-Elut C18柱。提取后，胆汁酸可在4℃下使用Sep-Pak SIL柱分离为三个部分：未结合型、甘氨酸结合型和牛磺酸结合型，用乙醇 - 氯仿 - 水 - 冰醋酸混合物洗脱。从回收率、重现性和各部分之间的分离度方面对这些提取和分馏程序进行了评估。发现所有这些参数都令人满意。尽管发现Sep-Pak SIL柱上分馏的重现性在不同批次间有所不同，但这并未造成重大困难。使用所述程序进行提取和分馏后，通过毛细管气液色谱法对正常人和肝胆疾病患者的血浆进行了分析。
