基于晶体学的高通量蛋白质结构测定环境中的核磁共振技术。
NMR in a crystallography-based high-throughput protein structure-determination environment.
作者信息
Wüthrich Kurt
机构信息
Department of Molecular Biology and Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1365-6. doi: 10.1107/S1744309110030320. Epub 2010 Sep 30.
Abstract
An introduction is provided to three papers which compare corresponding protein crystal and NMR solution structures determined by the Joint Center for Structural Genomics (JCSG). Special mention is made of the JCSG strategy for combined use of the two techniques, and of potential applications of the concept of `reference crystal structures', which is introduced in the following three papers.
摘要
本文介绍了三篇论文，这些论文比较了由结构基因组学联合中心（JCSG）测定的相应蛋白质晶体结构和核磁共振溶液结构。特别提到了JCSG联合使用这两种技术的策略，以及在以下三篇论文中引入的“参考晶体结构”概念的潜在应用。
相似文献
1
NMR in a crystallography-based high-throughput protein structure-determination environment.基于晶体学的高通量蛋白质结构测定环境中的核磁共振技术。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1365-6. doi: 10.1107/S1744309110030320. Epub 2010 Sep 30.
2
NMR in structural genomics to increase structural coverage of the protein universe: Delivered by Prof. Kurt Wüthrich on 7 July 2013 at the 38th FEBS Congress in St. Petersburg, Russia.结构基因组学中的核磁共振技术以增加蛋白质宇宙的结构覆盖率：由库尔特·维特里希教授于2013年7月7日在俄罗斯圣彼得堡举行的第38届欧洲生物化学学会联合会大会上发表。
FEBS J. 2016 Nov;283(21):3870-3881. doi: 10.1111/febs.13751. Epub 2016 Jun 9.
3
APSY-NMR for protein backbone assignment in high-throughput structural biology.用于高通量结构生物学中蛋白质主链归属的APSY-NMR
J Biomol NMR. 2015 Jan;61(1):47-53. doi: 10.1007/s10858-014-9881-8. Epub 2014 Nov 27.
4
NMR structure of the protein NP_247299.1: comparison with the crystal structure.蛋白质NP_247299.1的核磁共振结构：与晶体结构的比较。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1367-80. doi: 10.1107/S1744309110005890. Epub 2010 Jul 6.
5
Comparison of NMR and crystal structures for the proteins TM1112 and TM1367.蛋白质TM1112和TM1367的核磁共振（NMR）结构与晶体结构比较。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1381-92. doi: 10.1107/S1744309110020956. Epub 2010 Aug 7.
6
Structure determination using solution NMR: Is it worth the effort?使用溶液 NMR 进行结构测定：是否值得付出努力？
J Magn Reson. 2019 Sep;306:195-201. doi: 10.1016/j.jmr.2019.07.045. Epub 2019 Jul 13.
7
High-throughput three-dimensional protein structure determination.高通量三维蛋白质结构测定
Curr Opin Biotechnol. 2001 Aug;12(4):348-54. doi: 10.1016/s0958-1669(00)00226-3.
8
Comparison of NMR and crystal structures of membrane proteins and computational refinement to improve model quality.膜蛋白的核磁共振（NMR）结构与晶体结构比较以及用于提高模型质量的计算优化
Proteins. 2018 Jan;86(1):57-74. doi: 10.1002/prot.25402. Epub 2017 Nov 8.
9
NMR spectroscopy and protein structure determination: applications to drug discovery and development.核磁共振光谱学与蛋白质结构测定：在药物发现与开发中的应用
Curr Pharm Biotechnol. 2005 Apr;6(2):105-20. doi: 10.2174/1389201053642367.
10
Advances in protein NMR provided by the NIGMS Protein Structure Initiative: impact on drug discovery.美国国立综合医学科学研究所蛋白质结构计划带来的蛋白质核磁共振技术进展：对药物发现的影响。
Curr Opin Drug Discov Devel. 2010 May;13(3):335-49.
引用本文的文献
1
NMR in structural genomics to increase structural coverage of the protein universe: Delivered by Prof. Kurt Wüthrich on 7 July 2013 at the 38th FEBS Congress in St. Petersburg, Russia.结构基因组学中的核磁共振技术以增加蛋白质宇宙的结构覆盖率：由库尔特·维特里希教授于2013年7月7日在俄罗斯圣彼得堡举行的第38届欧洲生物化学学会联合会大会上发表。
FEBS J. 2016 Nov;283(21):3870-3881. doi: 10.1111/febs.13751. Epub 2016 Jun 9.
2
The J-UNIO protocol for automated protein structure determination by NMR in solution.《溶液中通过 NMR 自动化蛋白质结构测定的 J-UNIO 方案》
J Biomol NMR. 2012 Aug;53(4):341-54. doi: 10.1007/s10858-012-9645-2. Epub 2012 Jul 3.
3
Structure and behavior of human α-thrombin upon ligand recognition: thermodynamic and molecular dynamics studies.人凝血酶α在配体识别时的结构和行为：热力学和分子动力学研究。
PLoS One. 2011;6(9):e24735. doi: 10.1371/journal.pone.0024735. Epub 2011 Sep 14.
4
The JCSG high-throughput structural biology pipeline.JCSG高通量结构生物学流程。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1137-42. doi: 10.1107/S1744309110038212. Epub 2010 Sep 30.
本文引用的文献
1
Comparison of NMR and crystal structures highlights conformational isomerism in protein active sites.核磁共振（NMR）结构与晶体结构的比较突出了蛋白质活性位点中的构象异构现象。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1393-405. doi: 10.1107/S1744309110033658. Epub 2010 Sep 30.
2
Comparison of NMR and crystal structures for the proteins TM1112 and TM1367.蛋白质TM1112和TM1367的核磁共振（NMR）结构与晶体结构比较。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1381-92. doi: 10.1107/S1744309110020956. Epub 2010 Aug 7.
3
NMR structure of the protein NP_247299.1: comparison with the crystal structure.蛋白质NP_247299.1的核磁共振结构：与晶体结构的比较。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Oct 1;66(Pt 10):1367-80. doi: 10.1107/S1744309110005890. Epub 2010 Jul 6.
4
NMR structure determination for larger proteins using backbone-only data.使用仅包含骨架数据的方法进行较大蛋白质的 NMR 结构测定。
Science. 2010 Feb 19;327(5968):1014-8. doi: 10.1126/science.1183649. Epub 2010 Feb 4.
5
Millisecond timescale fluctuations in dihydrofolate reductase are exquisitely sensitive to the bound ligands.二氢叶酸还原酶的毫秒时间尺度波动对结合配体极其敏感。
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1373-8. doi: 10.1073/pnas.0914163107. Epub 2010 Jan 8.
6
Hidden alternative structures of proline isomerase essential for catalysis.脯氨酸异构酶的隐藏替代结构对催化作用至关重要。
Nature. 2009 Dec 3;462(7273):669-73. doi: 10.1038/nature08615.
7
De novo protein structure generation from incomplete chemical shift assignments.从不完整的化学位移归属进行从头蛋白质结构生成。
J Biomol NMR. 2009 Feb;43(2):63-78. doi: 10.1007/s10858-008-9288-5. Epub 2008 Nov 26.
8
APSY-NMR with proteins: practical aspects and backbone assignment.蛋白质的非均匀采样核磁共振：实践要点与主链归属
J Biomol NMR. 2008 Nov;42(3):179-95. doi: 10.1007/s10858-008-9266-y. Epub 2008 Oct 8.
9
Automated amino acid side-chain NMR assignment of proteins using (13)C- and (15)N-resolved 3D [ (1)H, (1)H]-NOESY.使用（13）C和（15）N解析的3D [（1）H，（1）H]-NOESY对蛋白质进行自动氨基酸侧链核磁共振归属
J Biomol NMR. 2008 Sep;42(1):23-33. doi: 10.1007/s10858-008-9259-x. Epub 2008 Aug 16.
10
Automated sequence-specific protein NMR assignment using the memetic algorithm MATCH.使用混合算法MATCH进行自动化的序列特异性蛋白质核磁共振归属
J Biomol NMR. 2008 Jul;41(3):127-38. doi: 10.1007/s10858-008-9243-5. Epub 2008 May 30.
Zotero 插件