Departamento de Morfologia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil.
Mem Inst Oswaldo Cruz. 2010 Sep;105(6):834-7. doi: 10.1590/s0074-02762010000600018.
We have previously demonstrated selection favoring the JG strain of Trypanosoma cruzi in hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.
我们之前已经证明，在慢性感染等量混合的单克隆 JG 株和哥伦比亚株克隆 Col1.7G2 的 BALB/c 小鼠心脏中，选择有利于克氏锥虫 JG 株。为了评估细胞入侵效率是否驱动这种选择，我们使用这些相同的克氏锥虫种群感染 BALB/c 心肌细胞的原代培养物。与预期相反，Col1.7G2 寄生虫比 JG 寄生虫更大量地入侵心脏细胞培养物；然而，JG 寄生虫的细胞内繁殖比 Col1.7G2 寄生虫更有效。这种现象仅在心肌细胞中观察到，而在培养的 Vero 细胞中则没有观察到。双重感染（Col1.7G2+JG）显示出类似的结果。尽管入侵可能会影响组织选择，但我们的数据强烈表明，细胞内发育对于确定寄生虫的组织嗜性很重要。
